Visualization of adherent micro-organisms using different techniques

Author:

Hannig Christian1,Follo Marie2,Hellwig Elmar1,Al-Ahmad Ali1

Affiliation:

1. Department of Operative Dentistry and Periodontology, University of Freiburg, Hugstetter Str. 55, D-79106 Freiburg, Germany

2. Department of Hematology and Oncology, Core Facility, Albert-Ludwig University, Freiburg, Germany

Abstract

The visualization and quantification of adherent bacteria is still one of the most relevant topics in microbiology. Besides electron microscopic techniques such as transmission electron microscopy, scanning electron microscopy and environmental scanning electron microscopy, modern fluorescence microscopic approaches based on fluorogenic dyes offer detailed insight into bacterial biofilms. The aim of the present review was to provide an overview of the advantages and disadvantages of different methods for visualization of adherent bacteria with a special focus on the experiences gained in dental research.

Publisher

Microbiology Society

Subject

Microbiology (medical),General Medicine,Microbiology

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