Affiliation:
1. Department of Biology and Biotechnology, An-Najah National University, Nablus, Palestine
2. Central Veterinary Laboratory, Directorate of Veterinary Services and Animal Health, Ministry of Agriculture, Palestine
Abstract
Antibiotic resistance of Escherichia coli isolated from urinary tract infections (UTIs) is increasing worldwide. A total of 41 E. coli isolates were obtained from urine samples from hospitalized patients with a UTI in three hospitals in the northern districts of the West Bank, Palestine during March and June 2011. Resistance rates were: erythromycin (95 %), trimethoprim–sulfamethoxazole (59 %), ciprofloxacin (56 %), gentamicin (27 %), imipenem (22 %), amoxicillin (93 %), amoxicillin–clavulanic acid (32 %), ceftazidime (66 %) and cefotaxime (71 %). No meropenem-resistant isolates were identified in this study. Among the isolates, phylogenetic group B2 was observed in 13 isolates, D in 12 isolates, A in 11 isolates and B1 in five isolates. Thirty-five of the isolates were positive for an extended-spectrum β-lactamase phenotype. Among these isolates, the bla
CTX-M gene was detected in 25, and eight harboured the bla
TEM gene. None of the isolates contained the bla
SHV gene. Transformation experiments indicated that some of the β-lactamase genes (i.e. bla
CTX-M and bla
TEM) with co-resistance to erythromycin and gentamicin were plasmid encoded and transmissible. Apart from this, enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) revealed that the 41 isolates were genetically diverse and comprised a heterogeneous population with 11 ERIC-PCR profiles at a 60 % similarity level.
Subject
Microbiology (medical),General Medicine,Microbiology
Reference25 articles.
1. Molecular detection and epidemiology of extended-spectrum beta-lactamase genes prevalent in clinical isolates of Klebsiella pneumoniae and E. coli from Trinidad and Tobago;Akpaka;West Indian Med J,2010
2. Resistance trends and risk factors of extended spectrum β-lactamases in Escherichia coli infections in Aleppo, Syria
3. High prevalence of multidrug resistance in bacterial uropathogens from Kathmandu, Nepal
4. Urinary tract pathogens and resistance pattern
5. Performance Standards for Antimicrobial Susceptibility Testing; 23rd Informational Supplement M100-S23,2013
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