Microbacterium neimengense sp. nov., isolated from the rhizosphere of maize

Abstract

A Gram-positive, non-motile, non-spore-forming, rod-shaped bacterium, strain 7087T, was isolated from rhizosphere of maize in China. The strain grew at 4–50 °C and at pH 4–10, with optima of 37 °C and pH 7.0, respectively. Phylogenetic analysis based on the full-length 16S rRNA gene sequence revealed that strain 7087T was a member of the genus Microbacterium . High levels of 16S rRNA gene similarities were found between strain 7087T and Microbacterium binotii DSM 19164T (99.8 %). However, the DNA–DNA hybridization value between strain 7087T and Microbacterium binotii DSM 19164T was 24.2 %. The DNA G+C content of strain 7087T was 69.9 mol%. The major fatty acids were anteiso-C17 : 0 (36.45 %), anteiso-C15 : 0 (36.08 %) and iso-C16 : 0 (16.11 %). The predominant menaquinones were MK-10 (28.1 %), MK-11 (54.8 %) and MK-12 (17.1 %). The diagnostic diamino acid in the cell-wall peptidoglycan was ornithine. The major polar lipids are diphosphatidylglycerol, unknown phospholipids, an unknown glycolipid and unknown amino lipids. On the basis of these results, strain 7087T is considered to represent a novel species of the genus Microbacterium , for which the name Microbacterium neimengense sp. nov. is proposed. The type strain is 7087T ( = ACCC 03008T = DSM 24985T).