Brevibacterium yomogidense sp. nov., isolated from a soil conditioner made from poultry manure

Abstract

A novel Gram-stain-positive rod-shaped actinobacterium was isolated from a soil conditioner made from poultry manure. The isolate, designated strain MN-6-aT, contained anteiso-C15 : 0 and anteiso-C17 : 0 as the major fatty acids, and MK-7(H2) and MK-8(H2) as the major menaquinones. Phosphatidylglycerol was a major polar lipid. The G+C content of the genomic DNA was 67.4 mol%. Phylogenetic analysis showed that strain MN-6-aT was closely related to Brevibacterium salitolerans TRM 415T with 97.1 % 16S rRNA gene sequence similarity. DNA–DNA hybridization showed that strain MN-6-aT had 10.2 % genomic relatedness with B. salitolerans TRM 415T. On the basis of phenotypic, phylogenetic and chemotaxonomic data obtained in this study, strain MN-6-aT represents a novel species of the genus Brevibacterium , for which the name Brevibacterium yomogidense sp. nov. is proposed. The type strain is MN-6-aT ( = JCM 17779T = DSM 24850T).