Chryseolinea serpens gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from soil

Abstract

An aerobic chemoheterotrophic gliding bacterium, designated RYGT, was isolated from a soil in Germany. Cells were Gram-stain-negative, thin rods (0.4–0.6 µm in width and 2.0–5.5 µm in length). Cells multiplied by normal cell division and no resting stages were observed. Colonies were yellow and displayed swarming edges. Gliding motility was observed in wet mounts. Strain RYGT grew at pH 5.6–7.7 (optimum pH 6.6–7.0), at 13–37 °C (optimum 25–30 °C) and with 0–1.0 % NaCl (optimum 0–0.1 %). The isolate was incapable of atmospheric nitrogen fixation and grew on most mono- and disaccharides as well as a few polysaccharides and organic acids. The predominant menaquinone was MK-7, the major cellular fatty acids were C16 : 1ω5c and iso-C15 : 0 and the major intact polar lipids were composed of phosphatidylethanolamine derivatives and two unknown series. The DNA G+C content was 49.9 mol%. Based on 16S rRNA gene sequence analysis, the isolate belonged to the phylum Bacteroidetes , class Cytophagia , order Cytophagales , but was only distantly related to any cultured bacteria. The closest relatives were Ohtaekwangia koreensis 3B-2T and Ohtaekwangia kribbensis 10AOT (both 93 % 16S rRNA gene sequence similarity). We propose a novel genus and species, Chryseolinea serpens gen. nov., sp. nov.. Strain RYGT ( = DSM 24574T = ATCC BAA-2075T) is the type strain.