Affiliation:
1. Department of Microbiology and Immunology, University of Western Ontario, London, ON N6A 5C1, Canada
2. Department of Plant Pathology and Microbiology, The Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
Abstract
The life cycle, prey range and taxonomic status of aBdellovibrio-like organism, strain JSST, were studied. Strain JSSTwas isolated from sewage in London, Ontario, Canada, in enrichment culture withCaulobacter crescentusprey cells. During predation, this strain remained attached to the outside of a stalkedC. crescentuscell. No periplasmic growth stage was observed and no bdelloplast was formed. The stalked cells ofC. crescentusretained their shape and, after predation, were devoid of cytoplasmic content, as shown by transmission electron microscopy. A periplasmic growth stage has been a definitive character in the description of members of the generaBdellovibrio,Bacteriovorax,BacteriolyticumandPeredibacter. This is the first description of an epibiotic predator in this group of prokaryotic predators. The G+C content of the genomic DNA of strain JSSTwas 46.1 mol%. 16S rRNA gene sequence analysis showed that this strain was related toBdellovibrio bacteriovorusstrains HD100T, 109J, 114 and 127 (90–93 % similarity). Phylogenetic analysis based on 16S rRNA gene sequences grouped strain JSSTwith theBdellovibriocluster, but at a distance from otherBdellovibrioisolates. On the basis of features of the life cycle and phylogenetic data, it was concluded that strain JSSTmerits classification as the type strain of a novel species, for which the nameBdellovibrio exovorussp. nov. is proposed (type strain JSST = ATCC BAA-2330T = DSM 25223T).
Funder
Natural Sciences and Engineering Research Council of Canada
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
61 articles.
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