Lutibaculum baratangense gen. nov., sp. nov., a proteobacterium isolated from a mud volcano

Abstract

A novel Gram-negative, oval to rod-shaped, motile bacterium, strain AMV1T, was isolated from a soil sample collected from a mud volcano of Baratang Island, Andamans, India. The predominant fatty acids were C16 : 0 (5.7 %), C18 : 1ω7c (78.6 %) and C19 : 0 cyclo ω8c (6.3 %). Strain AMV1T contained ubiquinone 10 (Q-10) as the major respiratory quinone and minor quantities of ubiquinone 9 (Q-9). The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unidentified lipids, one unidentified phospholipid and one unidentified aminolipid. 16S rRNA gene sequence analysis indicated that strain AMV1T was related most closely to the type strains of Tepidamorphus gemmatus , Bauldia consociata , Afifella pfennigii and Amorphus coralli , four members of the order Rhizobiales (class Alphaproteobacteria ), with pairwise sequence similarities of 95.0, 94.5, 94.4 and 94.0 %, respectively; it shared <94 % 16S rRNA gene sequence similarity with all the other members of the order Rhizobiales . Phylogenetic analyses indicated that strain AMV1T clustered with Tepidamorphus gemmatus and with species of the genera Amorphus , Rhodobium and Afifella . Phenotypic and phylogenetic characteristics thus suggest that strain AMV1T is a representative of a novel species of a new genus, for which the name Lutibaculum baratangense gen. nov., sp. nov. is proposed. The type strain of Lutibaculum baratangense is AMV1T ( = KCTC 22669T = NBRC 105799T = CCUG 58046T).