Lactobacillus pasteurii sp. nov. and Lactobacillus hominis sp. nov.

Abstract

Strains 1517T and 61DT were characterized by phenotypic and molecular taxonomic methods. These Gram-positive lactic acid bacteria were homo-fermentative, facultatively anaerobic short rods. They were phylogenetically related to the genus Lactobacillus according to 16S rRNA gene sequence analysis, with 99 % similarity between strain 1517T and the type strain of Lactobacillus gigeriorum , and 98.6, 98.5 and 98.4 % between strain 61DT and Lactobacillus gasseri , Lactobacillus taiwanensis and Lactobacillus johnsonii , respectively. Multilocus sequence analysis and metabolic analysis of both strains showed variation between the two strains and their close relatives, with variation in the position of the pheS and rpoA genes. The DNA–DNA relatedness of 43.5 % between strain 1517T and L. gigeriorum , and 38.6, 29.9 and 39.7 % between strain 61DT and L. johnsonii , L. taiwanensis and L. gasseri , respectively, confirmed their status as novel species. Based on phenotypic and genotypic characteristics, two novel species of Lactobacillus are proposed: Lactobacillus pasteurii sp. nov., with 1517T ( = CRBIP 24.76T = DSM 23907T) as the type strain, and Lactobacillus hominis sp. nov., with 61DT ( = CRBIP 24.179T = DSM 23910T) as the type strain.