Budvicia diplopodorum sp. nov. and emended description of the genus Budvicia

Abstract

A Gram-negative, rod-shaped, weakly motile, non-spore-forming bacterium (D9T) was isolated from the gut of Cylindroiulus fulviceps (Diplopoda) on 1/3-strength nutrient agar plates. On the basis of 16S rRNA gene sequence similarity, strain D9T was shown to be phylogenetically closely related to the type strain of Budvicia aquatica , the sole species of the genus Budvicia , family Enterobacteriaceae . The similarity of the 16S rRNA gene sequences of strain D9T and B. aquatica DSM 5075T was 98.4 %. Other strains that showed high pairwise similarities with the isolate belonged to the genus Yersinia : Y. frederiksenii ATCC 33641T (96.8 % 16S rRNA gene sequence similarity), Y. massiliensis CCUG 53443T (96.8 %), Y. pestis NCTC 5923T (96.8 %), Y. pseudotuberculosis ATCC 29833T (96.8 %), Y. similis CCUG 52882T (96.7 %) and Y. ruckeri ATCC 29473T (96.5 % ). The similarities of sequences of the housekeeping genes rpoB, hsp60 and gyrB between strain D9T and B. aquatica DSM 5075T and other members of the Enterobacteriaceae were less than 94 %. Phylogenetic trees based on all four gene sequences unequivocally grouped the isolate with the type strain of B. aquatica and separately from the genus Yersinia . Cells contained the quinones Q-8, Q-7 and MK-8. The major polar lipids were phosphatidylglycerol and phosphatidylethanolamine. The G+C content of the DNA (48.3 mol%) and the whole-cell fatty acid composition of strain D9T (C14 : 0, C16 : 1ω7c, C16 : 0, cyclo-C17 : 0 and C18 : 1ω7c as major components) were typical for members of the Enterobacteriaceae . DNA–DNA hybridization of strain D9T with B. aquatica DSM 5075T resulted in a relatedness of 30.4 %, indicating that the isolate did not belong to B. aquatica . Physiological tests allowed the phenotypic differentiation of strain D9T from B. aquatica DSM 5075T as well as from members of the genus Yersinia . From these results, it is concluded that strain D9T represents a novel species, for which the name Budvicia diplopodorum sp. nov. is proposed (type strain D9T  = DSM 21983T  = CCM 7845T). The description of the genus Budvicia is emended.