Nonomuraea fuscirosea sp. nov., an actinomycete isolated from the rhizosphere soil of rehmannia (Rehmannia glutinosa Libosch)

Abstract

A novel actinomycete, designated strain NEAU-dht8T, was isolated from the rhizosphere soil of rehmannia (Rehmannia glutinosa Libosch) and characterized using a polyphasic approach. The organism was found to have morphological and chemotaxonomic characteristics typical of the genus Nonomuraea . The G+C content of the DNA was 68.47 mol%. On the basis of 16S rRNA gene sequence similarity studies, strain NEAU-dht8T was most closely related to Nonomuraea maheshkhaliensis 16-5-14T (99.31 %), Nonomuraea kuesteri GW 14-1925T (98.77 %), Nonomuraea coxensis JCM 13931T (98.71 %), Nonomuraea wenchangensis 210417T (98.44 %), Nonomuraea bangladeshensis 5-10-10T (98.36 %) and Nonomuraea salmonea DSM 43678T (98.0 %); similarities to other species of the genus Nonomuraea were lower than 98 %. Two tree-making algorithms based on 16S rRNA gene sequences showed that the isolate formed a phyletic line with its closest neighbour N. maheshkhaliensis 16-5-14T. However, the low level of DNA–DNA relatedness allowed the novel isolate to be differentiated from N. maheshkhaliensis 16-5-14T. Strain NEAU-dht8T could also be differentiated from other species of the genus Nonomuraea showing high 16S rRNA gene sequence similarity (98–98.77 %) by morphological and physiological characteristics. Thus, strain NEAU-dht8T is considered to represent a novel species of the genus Nonomuraea , for which the name Nonomuraea fuscirosea sp. nov. is proposed. The type strain is NEAU-dht8T ( = CGMCC 4.7104T = DSM 45880T).