Terriglobus aquaticus sp. nov., isolated from an artificial reservoir

Abstract

A pink-pigmented, chemo-organotrophic bacterium, designated strain 03SUJ4T, was isolated from the freshwater of Juam reservoir, Republic of Korea (35° 03′ 43′′ N 127° 14′ 15′′ E). Cells were aerobic, Gram-reaction-negative and non-motile rods. Strain 03SUJ4T grew at pH 6–7 (optimum, pH 6) and at 15–30 °C (optimum, 25 °C). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belonged to the genus Terriglobus , showing sequence similarities of 97.09 % and 96.82 % to Terriglobus roseus DSM 18391T and Terriglobus saanensis SP1PR4T, respectively. Low rpoB gene sequence similarity with members of the genus Terriglobus and different fingerprints with the repetitive primers BOX, ERIC and REP indicated that the isolate represented a novel species of the genus Terriglobus . The major cellular fatty acids were iso-C15 : 0, C16 : 0, C20 : 1ω9c, C14 : 0 and summed feature 3 (C16 : 1ω7c/C16 : 1ω6c). The DNA G+C content of strain 03SUJ4T was 63.2±0.1 mol% (mean±sd of three determinations). The predominant menaquinone was MK-8. The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and two unidentified phospholipids. Several phenotypic characteristics served to differentiate the novel isolate from recognized members of the genus Terriglobus . On the basis of the evidence presented in this study, a novel species, Terriglobus aquaticus sp. nov. is proposed for strain 03SUJ4T ( = KCTC 23332T = JCM 17517T).