Fluviicola hefeinensis sp. nov., isolated from the wastewater of a chemical factory

Abstract

A Gram-negative, strictly aerobic, yellow–orange-pigmented, motile, short rod-shaped, catalase-positive, oxidase-negative bacterium, strain MYL-8T, was isolated from wastewater of the Jin Tai Chemical Factory in Hefei, China. Strain MYL-8T grew optimally at 30 °C, in the absence of NaCl and at pH 7. Menaquinone 6 (MK-6) was the sole respiratory quinone and the major fatty acids were iso-C15 : 0, iso-C15 : 1 G, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH). The polar lipid profile was composed predominantly of unidentified polar lipids and aminolipids. Minor amounts of phosphatidylethanolamine and unidentified phospholipids were also detectable. The DNA G+C content of strain MYL-8T was 43.5 mol%. The 16S rRNA gene sequence of strain MYL-8T showed the highest similarity to that of Fluviicola taffensis RW 262T (97.03 %), followed by Wandonia haliotis Haldis-1-1T (92.05 %), Lishizhenia caseinilytica UST040201-001T (91.43 %) and Lishizhenia tianjinensis JCM 15141T (90.61 %). DNA–DNA relatedness between strain MYL-8T and F. taffensis RW 262T was 21.35±0.90 %. On the basis of phenotypic, chemotaxonomic, genomic and phylogenetic data, strain MYL-8T is considered to represent a novel species of the genus Fluviicola , for which the name Fluviicola hefeinensis sp. nov. is proposed. The type strain is MYL-8T ( = KACC 16597T = CCTCC AB 2013168T).