Novosphingobium malaysiense sp. nov. isolated from mangrove sediment

Abstract

A novel bacterium, strain MUSC 273T, was isolated from mangrove sediments of the Tanjung Lumpur river in the state of Pahang in peninsular Malaysia. The bacterium was yellow-pigmented, Gram-negative, rod-shaped and non-spore-forming. The taxonomy of strain MUSC 273T was studied by a polyphasic approach and the organism showed a range of phenotypic and chemotaxonomic properties consistent with those of the genus Novosphingobium . The 16S rRNA gene sequence of strain MUSC 273T showed the highest sequence similarity to those of Novosphingobium indicum H25T (96.8 %), N. naphthalenivorans TUT562T (96.4 %) and N. soli CC-TPE-1T (95.9 %) and lower sequence similarity to members of all other species of the genus Novosphingobium . Furthermore, in phylogenetic analyses based on the 16S rRNA gene sequence, strain MUSC 273T formed a distinct cluster with members of the genus Novosphingobium . DNA–DNA relatedness of strain MUSC 273T to the type strains of the most closely related species, N. indicum MCCC 1A01080T and N. naphthalenivorans DSM 18518T, was 29.2 % (reciprocal 31.0 %) and 17 % (reciprocal 18 %), respectively. The major respiratory quinone was ubiquinone Q-10, the major polyamine was spermidine and the DNA G+C content was 63.3±0.1 mol%. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidyldimethylethanolamine, phosphatidylcholine and sphingoglycolipid. The major fatty acids were C18 : 1ω7c, C17 : 1ω6c, C16 : 0, C15 : 0 2-OH and C16 : 1ω7c. Comparison of BOX-PCR fingerprints indicated that strain MUSC 273T represented a unique DNA profile. The combined genotypic and phenotypic data showed that strain MUSC 273T represents a novel species of the genus Novosphingobium , for which the name Novosphingobium malaysiense sp. nov. is proposed. The type strain is MUSC 273T ( = DSM 27798T = MCCC 1A00645T = NBRC 109947T).