Paenalcaligenes suwonensis sp. nov., isolated from spent mushroom compost

Abstract

A bacterial strain, ABC02-12T, was isolated from spent mushroom compost, a waste product of button mushroom cultivation. Cells of the strain were Gram-stain-negative, catalase- and oxidase-positive, non-spore-forming, aerobic flagellated rods. Optimum growth occurred at 28 °C and pH 7.0. 16S rRNA gene sequence analysis showed that strain ABC02-12T shared the highest sequence similarities with Paenalcaligenes hominis CCUG 53761AT (96.0 %), Alcaligenes faecalis subsp. parafaecalis GT (95.7 %), Alcaligenes faecalis subsp. faecalis IAM 12369T (95.4 %) and Pusillimonas noertemannii BN9T (95.3 %). According to the phylogenetic tree, strain ABC02-12T formed a robust cluster with Paenalcaligenes hominis CCUG 53761AT and Paenalcaligenes hermetiae KBL009T. The quinone system was ubiquinone Q-8 with minor amounts of Q-7. The major fatty acids (>5 % of total fatty acids) were C16 : 0, C16 : 1ω6c and/or C16 : 1ω7c (summed feature 3), C18 : 1ω7c and/or C18 : 1ω6c (summed feature 8), C17 : 0 cyclo, and iso-C16 : 1 I, C14 : 0 3-OH and/or an unknown fatty acid (summed feature 2). The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unknown aminolipid. Putrescine was the principal polyamine, with small amounts of 2-hydroxyputrescine and cadaverine. On the basis of the evidence presented in this study, strain ABC02-12T is a representative of a novel species within the genus Paenalcaligenes , for which the name Paenalcaligenes suwonensis sp. nov. is proposed. The type strain is ABC02-12T ( = KACC 16537T = NBRC 108927T).