Cnuella takakiae gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from Takakia lepidozioides

Abstract

A Gram-staining-negative, rod-shaped and non-spore-forming bacterium, designated strain RG1-1T, was isolated from Takakia lepidozioides collected from Gawalong glacier in Tibet, China, and characterized by using a polyphasic taxonomic approach. The predominant fatty acids of strain RG1-1T were iso-C15 : 0 (19.8 %), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c, 17.0 %), C16 : 0 (9.9 %) and iso-C17 : 0 3-OH (9.4 %); its major polar lipids were phosphatidylethanolamine, four unidentified aminolipids, one unidentified phospholipid, one unidentified aminoglycolipid, one unidentified glycolipid, and three unidentified lipids. Strain RG1-1T contained MK-7 as the dominant menaquinone, and the G+C content of its genomic DNA was 49.1 mol%. Strain RG1-1T exhibited the highest 16S rRNA gene sequence similarity (91.8 %) with Flavisolibacter ginsengiterrae Gsoil 492T and Flavisolibacter ginsengisoli Gsoil 643T. Phylogenetic analysis showed that strain RG1-1T was a member of the family Chitinophagaceae , phylum Bacteroidetes . On the basis of 16S rRNA gene sequence analysis, and phenotypic and chemotaxonomic data, strain RG1-1T is considered to represent a novel species of a novel genus, for which the name Cnuella takakiae gen. nov., sp. nov. is proposed. The type strain is RG1-1T ( = CGMCC 1.12492T = DSM 26897T).