Flavobacterium ginsengisoli sp. nov., isolated from soil of a ginseng field

Abstract

A novel bacterial strain, designated DCY54T, was isolated from a field cultivated with ginseng in Yongin, Republic of Korea. Cells were Gram-reaction-negative, yellow-pigmented, rod-shaped, non-spore-forming, and strictly aerobic. They were motile by gliding and produced flexirubin-type pigments. Growth occurred optimally at 25–30 °C, at pH 5.0–7.0 and in the presence of 0–1 % NaCl. The 16S rRNA sequence analysis demonstrated that strain DCY54T was most closely related to Flavobacterium defluvii EMB117T (96.9 %). The only isoprenoid quinone of strain DCY 54T was menaquinone-6 (MK-6) and the major polar lipids were phosphatidylethanolamine, one unidentified aminolipid and one unidentified lipid. The major cellular fatty acids (>15 %) were iso-C15 : 0, summed feature 3 (comprising C16 : 1ω7c and/or iso-C15 : 0 2-OH) and C16 : 0. The DNA G+C content was 33.3 mol%. Phylogenetic inference and phenotypic data supported affiliation of strain DCY54T to the genus Flavobacterium . Several physiological and biochemical tests differentiated strain DCY54T from the species of the genus Flavobacterium with validly published names. On the basis of data from a polyphasic study, strain DCY54T represents a novel species of the genus Flavobacterium for which the name Flavobacterium ginsengisoli sp. nov. is proposed. The type strain is DCY54T ( = KCTC 23318T = JCM 17336T).