Affiliation:
1. Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS-CSIC, 30100 Campus de Espinardo, Murcia, Spain
Abstract
Urolithins are dibenzopyranone metabolites that exert anti-inflammatory activity in vivo and are produced by the gut microbiota from the dietary polyphenols ellagic acid (EA) and ellagitannins. However, the bacteria involved in this process remain unknown. We report here a novel bacterium, strain CEBAS 1/15PT, capable of metabolizing EA to urolithins, that was isolated from healthy human faeces and characterized by determining phenotypic, biochemical and molecular methods. The strain was related to
Gordonibacter pamelaeae
7-10-1-bT, the type and only reported strain of the only species of the genus
Gordonibacter,
with about 97 % 16S rRNA gene sequence similarity; they were both obligately anaerobic, non-spore-forming, Gram-stain-positive, short-rods/coccobacilli and metabolized only small numbers of carbon sources. l-Fucose, d-fructose, turanose, d-galacturonic acid and α-ketobutyric acid were metabolized by strain CEBAS 1/15PT, while
G. pamelaeae
was negative for metabolism of these compounds. The whole-cell fatty acids consisted predominantly of saturated fatty acids (70 %); strain CEBAS 1/15PT differed significantly from
G. pamelaeae
in the major fatty acid, which was C18 : 1ω9c, while anteiso-C15 : 0 was the major component for
G. pamelaeae
. The presence of a number of different fatty acid peaks, especially C19 : 0 cyclo and C18 : 1ω6c, was also indicative of distinct species. Six glycolipids (GL1–6) were recognized, while, in
G. pamelaeae,
only four glycolipids were described. On the basis of these data, the novel species Gordonibacter urolithinfaciens sp. nov. is described, with strain CEBAS 1/15PT ( = DSM 27213T = CCUG 64261T) as the type strain.
Funder
CICYT
Fundación Séneca de la Región de Murcia
Fun-C-Food
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
122 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献