Polaribacter huanghezhanensis sp. nov., isolated from Arctic fjord sediment, and emended description of the genus Polaribacter

Abstract

A Gram-negative, orange-colony-forming, aerobic and non-flagellated bacterium, designated strain SM1202T, was isolated from marine sediment of Kongsfjorden, Svalbard. Analysis of 16S rRNA gene sequences revealed that strain SM1202T was phylogenetically closely related to the genus Polaribacter . It shared the highest 16S rRNA gene sequence similarity with the type strain of Polaribacter dokdonensis (94.2 %) and 92.7–93.9 % sequence similarity with type strains of other known species of the genus Polaribacter . The strain grew at 4–35 °C and with 1.0–5.0 % (w/v) NaCl. It contained iso-C15 : 0, iso-C15 : 0 3-OH, iso-C13 : 0, C15 : 0, iso-C15 : 1 G, iso-C17 : 0 3-OH and C15 : 1ω6c as predominant cellular fatty acids and menaquinone-6 (MK-6) as the major respiratory quinone. The polar lipids of strain SM1202T were phosphatidylethanolamine, one unidentified lipid, two unidentified aminophospholipids and one unidentified aminolipid. The genomic DNA G+C content of strain SM1202T was 36.4 mol%. On the basis of the data from this polyphasic taxonomic study, strain SM1202T represents a novel species in the genus Polaribacter of the family Flavobacteriaceae , for which the name Polaribacter huanghezhanensis sp. nov. is proposed. The type strain of Polaribacter huanghezhanensis is SM1202T ( = CCTCC AB 2013148T = KCTC 32516T). An emended description of the genus Polaribacter is also presented.