Nocardioides salsibiostraticola sp. nov., isolated from biofilm formed in coastal seawater

Abstract

A Gram-staining-positive, non-motile, aerobic, non-spore-forming and short rod-shaped bacterial strain, PAMC 26527T, was isolated from biofilm formed in coastal seawater of the Norwegian Sea. Analysis of the 16S rRNA gene sequence of strain PAMC 26527T revealed a clear affiliation with the genus Nocardioides . Based on phylogenetic analysis, strain PAMC 26527T showed the closest phylogenetic relationship with Nocardioides caricicola YC6903T with 16S rRNA gene sequence similarity of 96.3 %. Strain PAMC 26527T grew in the presence of 0–5.0 % sea salts. The optimum temperature and pH for growth were 20 °C and pH 7.5. The major cellular fatty acids of strain PAMC 26527T were iso-C16 : 0, C17 : 1ω8c and C18 : 1ω9c and the major isoprenoid quinone was MK-8(H4). Cell-wall analysis showed that strain PAMC 26527T contained ll-diaminopimelic acid. The genomic DNA G+C content was 69.3 mol%. The combined phenotypic, chemotaxonomic and phylogenetic data showed that strain PAMC 26527T could be clearly distinguished from recognized members of the genus Nocardioides . Thus, strain PAMC 26527T should be classified as representing a novel species in the genus Nocardioides , for which the name Nocardioides salsibiostraticola sp. nov. is proposed. The type strain is PAMC 26527T ( = KCTC 29158T = JCM 18743T).