Flavobacterium aquaticum sp. nov., isolated from a water sample of a rice field

Abstract

Strain JC164T was isolated from a water sample from a rice field at Jamdih, Mau, Uttar Pradesh, India. Colonies of strain JC164T were brown–yellow and cells were Gram-stain-negative. Catalase, oxidase and amylase were present. iso-C15 : 0, iso-C16 : 0, iso-C15 : 1 G, iso-C15 : 0 3-OH and iso-C14 : 0 were the predominant fatty acids with minor amounts of iso-C16 : 0 3-OH, anteiso-C15 : 0, C16 : 0, iso-C16 : 1 H, iso-C14 : 0 3-OH and iso-C13 : 0. Strain JC164T contained phosphatidylethanolamine and a few unidentified lipids (L1, L3 and L6) as major polar lipids. Bacteriohopane derivative 1 (BHD1) and diplopterol (DPL) were the major hopanoids. β-Carotene was one among the several spirilloxanthin series carotenoids present in strain JC164T. Genomic DNA G+C content was 39.6 mol%. 16S rRNA gene sequence comparisons indicated that strain JC164T represents a member of the genus Flavobacterium (family Flavobacteriaceae , class Flavobacteriia ). The most closely related taxa to strain JC164T were Flavobacterium sasangense YC6274T (98.5 %), Flavobacterium cucumis R2A45-3T (98.1 %), Flavobacterium cheniae NJ-26T (97.2 %) and the novel strain possessed <95.1 % sequence similarity with other members of the genus Flavobacterium . However, strain JC164T showed 12.5±2, 13.6±1 and 17.4±2 % genomic DNA association (based on DNA–DNA hybridization) with Flavobacterium sasangense KCTC 22246T, Flavobacterium cucumis DSM 18830T and Flavobacterium cheniae CGMCC 1.6844T, respectively. The distinct genomic difference and morphological, physiological and chemotaxonomic differences from the previously described taxa support the classification of strain JC164T as a representative of a novel species of the genus Flavobacterium , for which the name Flavobacterium aquaticum sp. nov. is proposed. The type strain is JC164T ( = KCTC 32196T = CGMCC 1.12398 = LMG 27251T).