Tahibacter caeni sp. nov., isolated from activated sludge

Abstract

A Gram-reaction-negative, aerobic, rod-shaped, non-spore-forming, non-motile bacterial strain, designated BUT-6T, was isolated from activated sludge of a wastewater-treatment facility. The strain grew at 15–35 °C (optimum 30 °C), pH 4.0–10.0 (optimum pH 7.0) and 0–3.0 % (w/v) NaCl (optimum 1.0 %). Phylogenetic analysis based on 16S rRNA sequences showed that strain BUT-6T was most closely related to Tahibacter aquaticus PYM5-11T (98.6 % similarity). However, the DNA–DNA relatedness between strain BUT-6T and T. aquaticus PYM5-11T was 47.1 %. The major fatty acids (>10 % of total fatty acids) of strain BUT-6T were iso-C15 : 0, iso-C17 : 1ω9c and iso-C17 : 0. The major respiratory quinone was ubiquinone Q-8. The profile of polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, an unidentified aminophospholipid, three unknown aminolipids and unidentified phospholipids. The DNA G+C content of strain BUT-6T was 71.7 mol%. On the basis of the data from the polyphasic taxonomic study presented, strain BUT-6T is considered to represent a novel species of the genus Tahibacter , for which the name Tahibacter caeni sp. nov. is proposed. The type strain is BUT-6T ( = CCTCC AB 2013266T = KACC 17139T).