Characterization of 17 strains belonging to the Mycobacterium simiae complex and description of Mycobacterium paraense sp. nov.-Reference-Cited by-同舟云学术

Characterization of 17 strains belonging to the Mycobacterium simiae complex and description of Mycobacterium paraense sp. nov.

Published:2015-02-01 Issue:Pt_2 Volume:65 Page:656-662
ISSN:1466-5026
Container-title:International Journal of Systematic and Evolutionary Microbiology
language:en
Short-container-title:

Author:

Fusco da Costa Ana R.¹,Fedrizzi Tarcisio²,Lopes Maria L.¹,Pecorari Monica³,Oliveira da Costa Wana L.¹,Giacobazzi Elisabetta²,da Costa Bahia Jeann R.¹,De Sanctis Veronica²,Batista Lima Karla V.¹,Bertorelli Roberto²,Grottola Antonella³,Fabio Anna³,Mariottini Alessandro⁴,Ferretti Pamela²,Di Leva Francesca²,Fregni Serpini Giulia³,Tagliazucchi Sara³,Rumpianesi Fabio³,Jousson Olivier²,Segata Nicola²,Tortoli Enrico⁵

Affiliation:

1. Bacteriology and Mycology Section, Evandro Chagas Institute, Pará, Brazil

2. NGS Facility, Laboratory of Biomolecular Sequence and Structure Analysis for Health, Centre for Integrative Biology, University of Trento, Italy

3. Microbiology and Virology Unit, Policlinico University Hospital, Modena, Italy

4. Genetic Unit, Careggi University Hospital, Florence, Italy

5. Emerging Bacterial Pathogens Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy

Abstract

Fourteen mycobacterial strains isolated from pulmonary samples of independent patients in the state of Pará (Brazil), and three strains isolated in Italy, were characterized using a polyphasic approach. Thorough genetic investigation, including whole-genome sequencing, demonstrated that the strains belong to the

M. simiae

complex, being most closely related to

Mycobacterium interjectum

. For 14 of the strains, evidence emerged supporting their inclusion in a previously unreported species of the genus

Mycobacterium

, for which the name Mycobacterium paraense sp. nov. is proposed (type strain, IEC26T = DSM 46749T = CCUG 66121T). The novel species is characterized by slow growth, unpigmented or pale yellow scotochromogenic colonies, and a HPLC mycolic acid profile different from other known mycobacteria. In different genetic regions, high sequence microheterogeneity was detected.

Publisher

Microbiology Society

Subject

General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology

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