Endozoicomonas atrinae sp. nov., isolated from the intestine of a comb pen shell Atrina pectinata

Abstract

A novel bacterium, designated strain WP70T, was isolated from the gut of a comb pen shell (Atrina pectinata) collected from the southern sea of Yeosu in Korea. The isolate was Gram-stain-negative, aerobic, non-motile and rod-shaped. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain WP70T belonged to the genus Endozoicomonas . The highest level of sequence similarity (98.4 %) was shared with Endozoicomonas elysicola MKT110T. Optimal growth occurred in 2 % (w/v) NaCl at 30 °C and at pH 7. The major cellular fatty acids were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C16 : 0. The main respiratory quinone was Q-9. The polar lipids comprised phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, three unidentified phospholipids, an unidentified aminolipid, an unidentified aminophospholipid and an unidentified lipid. The genomic DNA G+C content was 50.5 mol% and DNA–DNA hybridization values indicated <11 % genomic relatedness to the closest species. Physiological, biochemical, chemotaxonomic and genotypic analyses indicated that strain WP70T represents a novel species of the genus Endozoicomonas , for which the name Endozoicomonas atrinae sp. nov. is proposed. The type strain is WP70T ( = KACC 17474T = JCM 19190T).