Affiliation:
1. Department of Life Science, Chung-Ang University, Seoul 156-756, Republic of Korea
Abstract
A Gram-staining-positive, aerobic, motile bacterium, designated strain MJ3T, was isolated from myeolchi-jeot (anchovy jeotgal), a traditional fermented seafood in South Korea. Cells were non-endospore-forming cocci showing catalase- and oxidase-positive reactions. Growth of strain MJ3T was observed at 15–45 °C (optimum, 30 °C), at pH 6.0–9.0 (optimum, pH 7.0–8.0) and in the presence of 1–24 % (w/v) NaCl (optimum, 10 % NaCl). Phylogenetic inference based on 16S rRNA gene sequences showed that strain MJ3T formed a tight phyletic lineage with members of the genus
Salimicrobium
. Strain MJ3T was related most closely to
Salimicrobium salexigens
29CMIT,
Salimicrobium album
DSM 20748T,
Salimicrobium flavidum
ISL-25T,
Salimicrobium luteum
BY-5T and
Salimicrobium halophilum
DSM 4771T, with similarities of 98.8 %, 98.7 %, 98.6 %, 98.4 % and 98.3 %, respectively. However, the DNA–DNA relatedness values between strain MJ3T (KF732837) and
S. salexigens
DSM 22782T,
S. album
DSM 20748T,
S. flavidum
DSM 23127T,
S. luteum
KCTC 3989T and
S. halophilum
JCM 12305T were 60±5.4 %, 58.5±6.5 %, 43.6±5.5 %, 37.2±5.8 % and 16.7±0.2 %, respectively. Chemotaxonomic data (sole isoprenoid quinone, MK-7; major cell-wall type, meso-diaminopimelic acid; major cellular fatty acids, anteiso-C15 : 0, anteiso-C17 : 0 and iso-C15 : 0; major polar lipids, phosphatidylglycerol and diphosphatidylglycerol; DNA G+C content, 46.3 mol%) also supported the affiliation of strain MJ3T with the genus
Salimicrobium
. Therefore, strain MJ3T represents a novel species of the genus
Salimicrobium
, for which the name Salimicrobium
jeotgali sp. nov. is proposed. The type strain is MJ3T ( = KACC 16972T = JCM 19758T).
Funder
Chung-Ang University
National Institute of Biological Resources (NIBR), Republic of Korea
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
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