Pedobacter rivuli sp. nov., isolated from a freshwater stream

Abstract

A Gram-staining-negative, rod-shaped, red strain, HME8457T, was isolated from a freshwater stream located in the Republic of Korea. A phylogenetic tree based on 16S rRNA gene sequences showed that strain HME8457T formed a lineage within the genus Pedobacter . Strain HME8457T was most closely related to species of the genus Pedobacter : Pedobacter daechungensis Dae 13T (96.4 % sequence similarity), Pedobacter lentus DS-40T (95.3 %), Pedobacter terricola DS-45T (94.9 %), Pedobacter glucosidilyticus 1-2T (94.2 %) and ‘Pedobacter soyangensis’ HME6451 (93.6 %). The major fatty acids were iso-C15 : 0 (28.8 %), summed feature 3 (comprising C16 : 1ω6c and/or C16 : 1ω7c; 21.7 %), iso-C17 : 0 3-OH (7.7 %) and anteiso-C15 : 0 (6.2 %). The only respiratory quinone was MK-7. Polar lipid analysis revealed the presence of phosphatidylethanolamine, one unidentified aminolipid and two unidentified polar lipids. Sphingolipid was present. The DNA G+C content was 33.3 mol%. On the basis of the evidence presented in this study, strain HME8457T represents a novel species of the genus Pedobacter , for which the name Pedobacter rivuli sp. nov. is proposed; the type strain is HME8457T ( = KACC 17312T = CECT 8291T).