Natribaculum breve gen. nov., sp. nov. and Natribaculum longum sp. nov., halophilic archaea isolated from saline soil

Author:

Liu Qin12,Ren Min12,Zhang Li-Li12

Affiliation:

1. Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, Alaer 843300, PR China

2. College of Life Science, Tarim University, Alaer 843300, PR China

Abstract

Two halophilic archaeal strains, TRM20010T and TRM20345T, were isolated from saline soil of the Lop Nur region in Xinjiang, north-west China. Cells from the two strains were pleomorphic rods, stained Gram-negative and produced red-pigmented colonies. Strains TRM20010T and TRM20345T were able to grow at 30–62 °C (optimum 37 °C), 0.9–5.1 M NaCl (optimum 2.6 and 3.4 M, respectively) and pH 6.0–10.0 (optimum pH 7.0−7.5) and neither strain required Mg2+ for growth. The major polar lipids of the two strains were phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), two glycolipids chromatographically identical to galactosyl mannosyl glucosyl diether (TGD-1) and disulfated mannosyl glucosyl diether (S2-DGD). Phylogenetic analysis based on 16S rRNA and rpoB′ genes revealed that strains TRM20010T and TRM20345T clustered together and formed a distinct clade separated from the related genera Halovivax , Haloterrigena , Halostagnicola , Natronolimnobius and Natrinema . The DNA G+C contents of strains TRM20010T and TRM20345T were 63.9 and 63.8 mol%, respectively. The DNA–DNA hybridization value between strain TRM20010T and strain TRM20345T was 42.8 %. The phenotypic, chemotaxonomic and phylogenetic properties suggested that strains TRM20010T and TRM20345T represent two novel species in a new genus within the family Halobacteriaceae , for which the names Natribaculum breve gen. nov., sp. nov. (type strain TRM20010T = CCTCC AB2013112T = NRRL B-59996T) and Natribaculum longum sp. nov. (type strain TRM20345T = CCTCC AB2013113T = NRRL B-59997T) are proposed.

Funder

Xinjiang Production & Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin

National High-tech R&D Program of China

Publisher

Microbiology Society

Subject

General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology

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