Novosphingobium aquiterrae sp. nov., isolated from ground water

Abstract

A Gram-staining-negative, strictly aerobic, non-motile, non-spore-forming, yellow and rod-shaped bacterium, designated E-II-3T, was isolated from ground water at Daejeon in Korea. Strain E-II-3T grew between 4 and 45 °C (optimal growth at 28 °C), between pH 6.0 and 9.0 (optimal growth at pH 7.5) and at salinities of 0–2.0 % (w/v) NaCl, growing optimally with 0.5 % (w/v) NaCl. On the basis of 16S rRNA gene sequence analysis, strain E-II-3T was shown to belong to the genus Novosphingobium and showed closest phylogenetic similarity to ‘Novosphingobium ginsenosidimutans’ FW-6 (97.7 %), Novosphingobium aromaticivorans F199T (96.9 %) and Novosphingobium subterraneum B0478T (96.5 %). The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and sphingoglycolipid. The predominant ubiquinone and polyamine components were Q-10 and spermidine, respectively. The major fatty acids were C18 : 1ω7c (34.0 %), C16 : 1ω7c and/or iso-C15 : 0 2-OH (23.8 %) and C17 : 1ω6c (19.3 %). The DNA G+C content of this novel isolate was 62.7 mol%. DNA–DNA relatedness between strain E-II-3T and ‘N. ginsenosidimutans’ KACC 16615, N. aromaticivorans KCTC 2888T and N. capsulatum KCTC 22844T was 38, 33 and 29 %, respectively. On the basis of polyphasic analysis from this study, strain E-II-3T represents a novel species of the genus Novosphingobium for which the name Novosphingobium aquiterrae sp. nov. is proposed. The type strain is E-II-3T ( = KACC 17599T = NBRC 109812T = NCAIM B 02537T).