First isolation of an Entomobirnavirus from free-living insects

Abstract

Drosophila X virus (DXV), the prototypeEntomobirnavirus, is a well-studied RNA virus model. Its origin is unknown, and so is that of the only other entomobirnavirus, Espirito Santo virus (ESV). We isolated an entomobirnavirus tentatively named Culex Y virus (CYV) from hibernatingCulex pipienscomplex mosquitoes in Germany. CYV was detected in three pools consisting of 11 mosquitoes each. Full-genome sequencing and phylogenetic analyses suggested that CYV and ESV define one sister species to DXV within the genusEntomobirnavirus. In contrast to the laboratory-derived ESV, the ORF5 initiation codon AUG was mutated to1927GUG in all three wild-type CYV isolates. Also in contrast to ESV, replication of CYV was not dependent on other viruses in insect cell culture. CYV could provide a wild-type counterpart in research fields relying on DXV and other cell culture-adapted strains.