Ochrobactrum cytisi sp. nov., isolated from nodules of Cytisus scoparius in Spain

Author:

Zurdo-Piñeiro José Luis1,Rivas Raúl1,Trujillo Martha E.1,Vizcaíno Nieves1,Carrasco José Antonio2,Chamber Manuel2,Palomares Antonio3,Mateos Pedro F.1,Martínez-Molina Eustoquio1,Velázquez Encarna1

Affiliation:

1. Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain

2. CIFA Las Torres, Alcalá del Río, Sevilla, Spain

3. Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Sevilla, Sevilla, Spain

Abstract

Two strains named ESC1T and ESC5 were isolated from nodules of Cytisus scoparius growing in a Spanish soil. Phylogenetic analysis of the 16S rRNA gene showed that these strains belong to the genus Ochrobactrum, their closest relatives being Ochrobactrum anthropi and Ochrobactrum lupini, with 100 and 99.9 % similarity to the respective type strains. Despite this high similarity, the results of DNA–DNA hybridization, phenotypic tests and fatty acid analyses showed that these strains represent a novel species of genus Ochrobactrum. The DNA–DNA hybridization values were respectively 70, 66 and 55 % with respect to O. lupini LUP21T, O. anthropi DSM 6882T and Ochrobactrum tritici DSM 13340T. The predominant fatty acids were C18 : 1 ω7c and C18 : 1 2-OH. Strains ESC1T and ESC5 were strictly aerobic and were able to reduce nitrate and to hydrolyse aesculin. They produced β-galactosidase and β-glucosidase and did not produce urease after 48 h incubation. The G+C content of strain ESC1T was 56.4 mol%. Both strains ESC1T and ESC5 contained nodD and nifH genes on megaplasmids that were related phylogenetically to those of rhizobial strains nodulating Phaseolus, Leucaena, Trifolium and Lupinus. From the results of this work, we propose that the strains isolated in this study be included in a novel species named Ochrobactrum cytisi sp. nov. The type strain is ESC1T (=LMG 22713T=CECT 7172T).

Publisher

Microbiology Society

Subject

General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology

Reference29 articles.

1. Basic local alignment search tool;Altschul;J Mol Biol,1990

2. The growth of Rhizobium in synthetic media;Bergersen;Aust J Biol,1961

3. A rapid method for the base ratio determination of bacterial DNA;Cashion;Anal Biochem,1977

4. The quantitative measurement of DNA hybridization from renaturation rates;De Ley;Eur J Biochem,1970

5. Determinative methods of light microscopy;Doetsch,1981

Cited by 110 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3