Fervidobacterium changbaicum sp. nov., a novel thermophilic anaerobic bacterium isolated from a hot spring of the Changbai Mountains, China

Abstract

A thermophilic, obligately anaerobic, rod-shaped bacterium (strain CBS-1T) was isolated from a hot spring mixture of water and mud of the Changbai Mountains, China. Strain CBS-1T was found to be non-sporulating, Gram-negative, with optimal growth at 75–80 °C. It grew on a wide range of carbon sources, including glucose, lactose, maltose, starch, sorbitol and pyruvate amongst others. The DNA G+C content of strain CBS-1T was 31.9 mol%. The 16S rRNA gene sequence analysis indicated that the strain was a member of the genus Fervidobacterium. The high concentration of C16 : 0 (52.2 %) in the fatty acid profile of the cell envelope supported its inclusion as a member of the genus Fervidobacterium. On the basis of the low values of DNA–DNA hybridization (25.8 and 20.5 %) and phenotypic features, strain CBS-1T represents a novel species of the genus Fervidobacterium, for which the name Fervidobacterium changbaicum sp. nov. is proposed. The type strain is CBS-1T (=DSM 17883T=JCM 13353T).