Brevibacterium samyangense sp. nov., an actinomycete isolated from a beach sediment

Abstract

A novel actinomycete, strain SST-8T, was isolated from sand sediment of Samyang Beach in Jeju, Korea, and subjected to a polyphasic taxonomic study. The organism, which produced opaque, circular, yellow colonies, with a coryneform morphology, showed the following chemotaxonomic characteristics:meso-diaminopimelic acid as the diamino acid in the peptidoglycan, MK-8(H2) as the major menaquinone, phosphatidylglycerol as the only polar lipid, anteiso-C15 : 0and anteiso-C17 : 0as major fatty acids and a DNA G+C content of 70.7 mol%. The combination of morphological and chemotaxonomic features supported its classification in the genusBrevibacterium. Phylogenetic analyses, based on 16S rRNA gene sequence studies, showed that strain SST-8Tformed an intermediate branch between theBrevibacterium luteolum/Brevibacterium otitidisandBrevibacterium mcbrellneri/Brevibacterium paucivoransclusters. Sequence similarity calculations based on a neighbour-joining analysis revealed that the closest relatives of strain SST-8Twere the type strains ofB. paucivorans(96.6 %),B. luteolum(96.5 %),B. mcbrellneri(96.3 %),Brevibacterium avium(96.0 %) andB. otitidis(95.9 %). Based on a broad set of phenotypic and genetic data, it was evident that the strain represents a novel species of the genusBrevibacterium. The nameBrevibacterium samyangensesp. nov. is proposed, with SST-8T(=NRRL B-41420T=KCCM 42316T) as the type strain.