Zinc- and arsenic-resistant strains of Thiobacillus ferrooxidans have increased copy numbers of chromosomal resistance genes

Author:

Kondratyeva Tamara F.1,Muntyan Lyudmila N.2,Karavaiko Grygory I.2

Affiliation:

1. Research Group of Genosystematics and Evolution of Bacteria, Institute of Microbiology, Russian Academy of Sciences,Moscow 117811, Prospekt 60-let Octyabrya, 7/2,Russia

2. Laboratory of Microbial Transformation of Minerals, Institute of Microbiology, Russian Academy of Sciences,Moscow 117811, Prospekt 60-let Octyabrya, 7/2,Russia

Abstract

SUMMARY Pulsed-field gel electrophoresis (PFGE) was used to examine chromosomal DNA from various strains of Thiobacillus ferrooxidans: these were the reference strain VKM-458, strains isolated from different environments and pilot plants for processing gold-bearing concentrates, and strains experimentally adapted to high zinc and arsenic concentrations in growth medium. The restriction endonuclease Xbal digested T. ferrooxidans VKM-458 chromosomal DNA into a number of fragments sufficient for identification of their size and calculation of the size of the entire genome (2855/pm44 kb). Restriction fragment length polymorphism of the chromosomal DNA in various strains suggests the usefulness of this approach for analysis of the diversity of T. ferrooxidans strains and for the study of strain stability under conditions of industrial utilization. A comparison of Xbal-restriction patterns in parent strains and in strains with acquired enhanced resistance to zinc or arsenic revealed amplification of certain fragments in the resistant strains, i.e. a 98 kb fragment in strain TFZ and a 28 kb fragment in strain VKM-458As2. We suggest that the enhanced resistance to toxic metals in T. ferrooxidans is gained through increase of the copy number of resistance genes and enhanced synthesis of proteins involved in resistance.

Publisher

Microbiology Society

Subject

Microbiology

Reference19 articles.

1. Genome analysis of Legionella ssp. by orthogonal field alternation gel electrophoresis (OFAGE).;Bender;FEMS Microbiol Lett,1990

2. An electrophoretic karyotype for yeast.;Carle;Proc Natl Acad Sci USA,1985

3. DNA restriction fingerprint analysis of the soil bacterium Azospirillum.;Giovannetti;J Gen Microbiol,1990

4. Genome analysis of Pseudomonas aeruginosa by field inversion gel electrophoresis.;Grothues;FEMS Microbiol Lett,1987

5. Adaptation of Thiobacillus ferrooxidans for industrial applications.;Holmes,1989

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3