The gene for Campylobacter trigger factor: evidence for multiple transcription start sites and protein products-Reference-Cited by-同舟云学术

The gene for Campylobacter trigger factor: evidence for multiple transcription start sites and protein products

Published:1995-06-01 Issue:6 Volume:141 Page:1359-1367
ISSN:1350-0872
Container-title:Microbiology
language:en
Short-container-title:

Author:

Griffiths Phillippa L.¹,Park Robert W. A.²,Connerton Ian F.³

Affiliation:

1. 1 Department of Biochemistry, Imperial College of Science, Technology and Medicine, Exhibition Road, London SW7 2AZ, UK

2. 2 Department of Microbiology, University of Reading, PO Box 228, Reading RG6 2AJ, UK

3. 3 Department of Protein Engineering, Institute of Food Research, Reading Laboratory, Earley Gate, Whiteknights Road, Reading RG6 2EF, UK

Abstract

A gene encoding a protein of apparent molecular mass 56 kDa that shares 31 % identity with the amino acid sequence of trigger factor from Escherichia coli (a protein thought to be involved in cell division), was cloned from Campylobacter jejuni NCTC 11168. The clone was selected from a lambda ZAP II genomic DNA library following an immuno-screen using antiserum raised against glycine-extractable proteins from C. jejuni. The gene has two potential initiation codons, giving rise to two possible nested protein products. Complex differential growth-phase-dependent transcripts give rise to these products.

Publisher

Microbiology Society

Subject

Microbiology

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