Pseudoxanthomonas indica sp. nov., isolated from a hexachlorocyclohexane dumpsite

Abstract

A bacterial strain, designated P15T, was isolated from the soil of an open hexachlorocyclohexane dumpsite. Comparative sequence analysis showed that strain P15T displayed high 16S rRNA gene sequence similarities (94.4–97.2 %) with members of the genus Pseudoxanthomonas. The isolate was most closely related to Pseudoxanthomonas mexicana AMX 26BT (97.2 % 16S rRNA gene sequence similarity) and Pseudoxanthomonas japonensis 12-3T (97.2 %). DNA–DNA relatedness studies showed unambiguously that strain P15T represented a novel species that was separate from P. mexicana DSM 17121T (7.7 %) and P. japonensis DSM 17109T (9.4 %). The predominant cellular fatty acids of strain P15T were iso-C16 : 0 (21.4 %), iso-C15 : 0 (16.1 %), summed feature 9 (comprising iso-C17 : 1ω9c and/or 10-methyl C16 : 0; 14.9 %), iso-C11 : 0 3-OH (8.3 %) and iso-C14 : 0 (7.0 %). The polar lipid profile of strain P15T showed the presence of large amounts of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol in addition to unknown glycolipids, phospholipids and an amino-group-containing polar lipid. Ubiquinone 8 was found as the major quinone. The polyamine profile showed the presence of spermidine. The DNA G+C content was 62.9±2 mol%. Strain P15T is described as representing a new member of the genus Pseudoxanthomonas, for which the name Pseudoxanthomonas indica sp. nov. is proposed. The type strain is P15T ( = MTCC 8596T = CCM 7430T).