Saccharomonospora marina sp. nov., isolated from an ocean sediment of the East China Sea

Abstract

A novel, aerobic, Gram-positive actinomycete strain, designated XMU15T, was isolated from an ocean sediment collected from Zhaoan Bay in the East China Sea and was subjected to a polyphasic approach to determine its taxonomic position. The isolate grew optimally at 28 °C and at pH 7.0 in the presence of 3 % (w/v) NaCl on ISP medium 2. Gelatin liquefaction, milk coagulation and nitrate reduction were positive. Cellulose and starch hydrolysis, hydrogen sulfide and melanin production, and catalase, urease and oxidase activities were negative. The predominant menaquinone of the isolate was MK-9 (H4), and meso-diaminopimelic acid was the diagnostic amino acid in the cell wall. The phospholipids of the isolate comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, and a minor amount of phosphatidylethanolamine. The major fatty acids of the strain were iso-C16 : 0 (26.36 %), C17 : 1 ω6c (16.80 %), C15 : 0 (16.2 %), C16 : 0 (8.90 %), C17 : 1 ω8c (7.69 %) and iso-C16 : 1 H (5.95 %). The DNA G+C content of the genomic DNA was 68.1 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that the isolate fell within the evolutionary radiation encompassed by the genus Saccharomonospora and showed the highest 16S rRNA gene sequence similarity (96.7 %) to Saccharomonospora xinjiangensis DSM 44391T. Based on the results of 16S rRNA gene sequence analysis and phenotypic and genotypic characterization, strain XMU15T (=KCTC 19701T =CCTCC AA 209048T) represents a novel species of the genus Saccharomonospora, for which the name Saccharomonospora marina sp. nov. is proposed.