Marmoricola scoriae sp. nov., isolated from volcanic ash

Abstract

A novel Gram-stain-positive, coccoid actinobacterium, designated strain Sco-D01T, was isolated from volcanic ash collected from Oreum (a parasitic volcanic cone) on Jeju Island, Republic of Korea. Cells were aerobic, oxidase-negative and catalase-positive. Colonies were vivid yellow, circular, smooth and convex. The diagnostic diamino acid in the cell wall was ll-diaminopimelic acid. The predominant menaquinone was MK-8(H4). The polar lipids were phosphatidylinositol, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and an unknown phospholipid. The fatty acid profile was represented by large amounts of saturated, unsaturated, 10-methyl and hydroxyl components. The DNA G+C content of strain Sco-D01T was 72.0 mol%. 16S rRNA gene sequence analysis revealed that strain Sco-D01T belonged to the family Nocardioidaceae and formed a distinct sublineage within the radiation of the genus Marmoricola. The level of DNA–DNA relatedness between strain Sco-D01T and its closest phylogenetic relative, Marmoricola aurantiacus DSM 12652T, was 30.2 % (35.4 % in duplicate measurements). On the basis of phenotypic and DNA–DNA hybridization data, strain Sco-D01T is considered to represent a novel species of the genus Marmoricola, for which the name Marmoricola scoriae sp. nov. is proposed. The type strain is Sco-D01T (=KCTC 19597T=DSM 22127T).