Haladaptatus cibarius sp. nov., an extremely halophilic archaeon from seafood, and emended description of the genus Haladaptatus

Abstract

A novel, extremely halophilic archaeon, D43T, was isolated from traditional salt-fermented seafood in Korea. The cells were Gram-negative-staining and motile. The strain grew at 15–50 °C, 10–30 % (w/v) NaCl and pH 6.0–8.0. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain D43Tis affiliated with the familyHalobacteriaceaein the domainArchaeaand had 95.5 % 16S rRNA gene sequence similarity withHaladaptatus paucihalophilusDX253T. The sequence from strain D43Tformed a clade with those fromHap. paucihalophilusregardless of which tree-generating algorithm was used. DNA–DNA hybridization experiments showed 25.8 % relatedness between the isolate andHap. paucihalophilusKCTC 4006T. Major lipids were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two unidentified glycolipids. The DNA G+C content of the isolate was 56.5 mol%. On the basis of this polyphasic taxonomic study, strain D43Trepresents a novel species in the genusHaladaptatus, for which the nameHaladaptatus cibariussp. nov. is proposed. The type strain is D43T(=DSM 19505T=JCM 15962T).