Affiliation:
1. Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi 110 007, India
2. DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstraße 7B, 38124 Braunschweig, Germany
Abstract
A Gram-stain-positive, aerobic, rod-shaped, yellow actinobacterium, designated MNA2T, was isolated from a hexachlorocyclohexane-contaminated soil in North India. Strain MNA2T showed 95 % 16S rRNA gene sequence similarity with Microbacterium halotolerans YIM 70130T. Phylogenetic analysis of 16S rRNA gene sequences showed that strain MNA2T belonged to a clade represented by the genus Microbacterium of the family Microbacteriaceae. Strain MNA2T contained anteiso-C15 : 0, anteiso-C17 : 0, iso-C16 : 0 and iso-C15 : 0 as the predominant fatty acids and diphosphatidylglycerol, phosphatidylglycerol and two unknown polar lipids. The menaquinones were MK-12, MK-11, MK-13 and MK-10, in an approximate molar ratio of 45 : 40 : 13 : 3, respectively. The DNA G+C content was 65.3 mol%. The peptidoglycan was of the B type of cross-linkage with ornithine as the diagnostic diamino acid. The results of the phylogenetic, phenotypic and chemotaxonomic analysis indicate that strain MNA2T belongs to a previously unrecognized species of the genus Microbacterium, for which the name Microbacterium lindanitolerans sp. nov. is proposed. The type strain is MNA2T (=DSM 22422T =CCM 7585T).
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Reference34 articles.
1. Taxonomic and serological studies on Micropolyspora faeni and Micropolyspora strains from soil bearing the specific epithet rectivirgula;Arden-Jones;J Gen Microbiol,1979
2. Description of Microbacterium foliorum sp. nov. and Microbacterium phyllosphaerae sp. nov., isolated from the phyllosphere of grasses and the surface litter after mulching the sward, and reclassification of Aureobacterium resistens (Funke et al. 1998) as Microbacterium resistens comb. nov;Behrendt;Int J Syst Evol Microbiol,2001
3. A rapid method of total lipid extraction and purification;Bligh;Can J Biochem Physiol,1959
4. Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment;Bruns;Int J Syst Evol Microbiol,2001
5. A rapid method for base ratio determination of bacterial DNA;Cashion;Anal Biochem,1977
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