Bacillus rigui sp. nov., isolated from wetland fresh water

Abstract

Two Gram-stain-positive strains, WPCB074Tand WPCB165, were isolated from fresh water collected from the Woopo wetland (Republic of Korea). Both strains were strictly aerobic, motile, endospore-forming rods. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strains WPCB074Tand WPCB165 belonged to the genusBacillusand that strain WPCB074Twas most closely related toBacillus solisalsiYC1T(98.4 % sequence similarity),B. barbaricusV2-BIII-A2T(97.7 %),B. macauensisZFHKF-1T(96.9 %),B. arsenicusCon a/3T(96.4 %) andB. gelatiniLMG 21880T(95.1 %). The 16S rRNA gene sequences of strains WPCB074Tand WPCB165 differed at one position (99.9 % similarity), suggesting that these two strains constitute a single species. DNA–DNA relatedness between strain WPCB074Tand the type strains ofB. solisalsi,B. barbaricus,B. macauensis,B. arsenicusandB. gelatiniwere 26, 17, 20, 14 and 7 %, respectively. Strain WPCB074Twas characterized by having cell-wall peptidoglycan based onmeso-diaminopimelic acid, MK-7 as the predominant menaquinone and iso-C15 : 0and anteiso-C15 : 0as the major fatty acids. The DNA G+C content of strain WPCB074Twas 41.9 mol%. On the basis of phenotypic properties, phylogeny and genomic distinctiveness, strain WPCB074Trepresents a novel species of the genusBacillusfor which the nameBacillus riguisp. nov. is proposed. The type strain is WPCB074T(=KCTC 13278T=JCM 16348T).