Tsukamurella soli sp. nov., isolated from soil

Abstract

A Gram-positive, rod-shaped, catalase-positive, oxidase-negative, white-coloured bacterium, designated strain JS18-1T, was isolated from a soil sample collected from Halla mountain, Jeju island, Korea. 16S rRNA gene sequence analysis revealed that the strain was most closely related to members of the genus Tsukamurella with levels of sequence similarity of 95.4–96.5 %. Strain JS18-1T shared highest 16S rRNA gene sequence similarity with Tsukamurella strandjordii DSM 44573T (96.5 %), Tsukamurella carboxydivorans Y2T (96.4 %) and Tsukamurella tyrosinosolvens DSM 44234T (96.4 %). The G+C content of the total DNA of strain JS18-1T was 70 mol%. The cell-wall peptidoglycan type was A1γ and mycolic acids were also detected. The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The major quinone was menaquinone-9 (MK-9) and major cell-wall sugars were arabinose, ribose and glucose. The major fatty acids (>10 % of the total fatty acids) were C16 : 0, C18 : 1 ω9c, C18 : 0 10-methyl and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2-OH). Phylogenetic analysis based on 16S rRNA gene sequences and chemotaxonomic, biochemical and physiological characteristics indicate that strain JS18-1T represents a novel species of the genus Tsukamurella, for which the name Tsukamurella soli sp. nov. is proposed. The type strain is JS18-1T (=KACC 20764T=DSM 45046T).