Sphingopyxis soli sp. nov., isolated from landfill soil

Abstract

A Gram-negative, aerobic, rod-shaped, motile, oxidase-positive, catalase-negative bacterium, designated strain BL03T, was isolated from landfill soil in Pohang, Republic of Korea. Colonies on Luria–Bertani agar plates were yellow. The strain grew in the presence of 0–3 % (w/v) NaCl, at 15–42 °C and at pH 5.0–9.5. The predominant ubiquinone was Q-10, and the major cellular fatty acids were C17 : 1ω6c, C15 : 02-OH and C18 : 1ω7c. Polar lipids detected were phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid and an unknown glycolipid. Spermidine was identified as the major polyamine component. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BL03Tbelongs to the genusSphingopyxiswith high sequence similarity toSphingopyxis taejonensisJSS54T(97.8 %),Sphingopyxis alaskensisRB2256T(97.4 %) andSphingopyxis chilensisS37T(96.9 %). Levels of DNA–DNA relatedness between strain BL03Tand the above three type strains were only 10.3–40.3 %. The DNA G+C content of strain BL03Twas 65.9 mol%. Based on the data presented, strain BL03Tis considered to represent a novel species of the genusSphingopyxis, for which the nameSphingopyxis solisp. nov. is proposed. The type strain is BL03T(=KCTC 22405T=JCM 15910T).