The formation of cyclopropane fatty acids in Salmonella enterica serovar Typhimurium

Abstract

The formation of cyclopropane fatty acid (CFA) and its role in the acid shock response inSalmonella entericaserovar Typhimurium (S. typhimurium) was investigated. Data obtained by GC/MS demonstrated that the CFA level inS. typhimuriumincreased upon its entry to the stationary phase, as in other bacteria. Thecfagene encoding CFA synthase was cloned, and mutants of thecfagene were constructed by allelic exchange. Acfamutant could not produce CFA and was sensitive to low pH. Introduction of a functionalcfagene into acfamutant cell made the mutant convert all unsaturated fatty acids to CFAs and partially restored resistance to low pH. Interestingly, the alternative sigma factor RpoS, which was induced during the stationary phase, affected the production of C19CFA but not C17CFA. Western blotting analysis showed that the increase in expression of CFA synthase at early stationary phase was due to the alternative sigma factor RpoS.