Lutibacter flavus sp. nov., a marine bacterium isolated from a tidal flat sediment

Abstract

A carotenoid-containing chemoheterotrophic bacterium, designated IMCC1507T, was isolated from a tidal flat sediment of the Yellow Sea, Korea. Strain IMCC1507T was Gram-negative, yellow, obligately aerobic, non-motile and flexirubin-negative. 16S rRNA gene sequence analysis indicated that strain IMCC1507T belonged to the genus Lutibacter in the family Flavobacteriaceae and exhibited 96.1–97.3 % 16S rRNA sequence similarity with the type strains of described species of the genus Lutibacter . DNA–DNA relatedness between strain IMCC1507T and Lutibacter litoralis KCCM 42118T ranged from 3.5±2.2 % to 11.2±2.4 %, indicating that strain IMCC1507T represented a novel genomic species in the genus Lutibacter . Chemotaxonomic characteristics of the isolate, i.e. the DNA G+C content (31.4 mol%), iso-C15 : 0 3-OH, iso-C15 : 0, iso-C15 : 1 G and anteiso-C15 : 0 as the major fatty acids, MK-6 as the predominant menaquinone and phosphatidylethanolamine as the major polar lipid, were consistent with its assignment to the genus Lutibacter . However, several phenotypic characteristics, including hydrolysis of macromolecules, enzyme activities and carbon source oxidation, differentiated strain IMCC1507T from members of the genus. Data from this study indicate that strain IMCC1507T represents a novel species in the genus Lutibacter , for which the name Lutibacter flavus sp. nov. is proposed. The type strain is IMCC1507T ( = KACC 14312T  = NBRC 107589T).