Description of Noviherbaspirillum malthae gen. nov., sp. nov., isolated from an oil-contaminated soil, and proposal to reclassify Herbaspirillum soli , Herbaspirillum aurantiacum , Herbaspirillum canariense and Herbaspirillum psychrotolerans as Noviherbaspirillum soli comb. nov., Noviherbaspirillum aurantiacum comb. nov., Noviherbaspirillum canariense comb. nov. and Noviherbaspirillum psychrotolerans comb. nov. based on polyphasic analysis

Abstract

An aerobic, Gram-negative, rod-shaped bacterium with polar flagella, strain CC-AFH3T, was isolated from an oil-contaminated site located in Kaohsiung county, Taiwan. Strain CC-AFH3T grew at 20–40 °C, pH 5.0–10.0 and <2 % (w/v) NaCl. 16S rRNA gene sequence analysis indicated that strain CC-AFH3T showed the greatest degree of similarity to Herbaspirillum soli SUEMI10T (96.5 %), H. aurantiacum SUEMI08T (96.3 %), H. canariense SUEMI03T (96.0 %), H. psychrotolerans PB1T (95.4 %) and members of other Herbaspirillum species (94.1–95.2 %), and lower similarity to members of other genera (<94 %). Phylogenetic analyses also positioned the novel strain in the genus Herbaspirillum as an independent lineage. The major fatty acids in strain CC-AFH3T were C10 : 0 3-OH, C12 : 0, C14 : 0 2-OH, C16 : 0, iso-C15 : 0 3-OH, C17 : 0 cyclo, C16 : 1ω7c/C16 : 1ω6c and C18 : 1ω7c/C18 : 1ω6c. The major polar lipids of strain CC-AFH3T were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The predominant quinone was ubiquinone 8 (Q-8) and the DNA G+C content was 63.4 mol%. On the basis of 16S rRNA gene sequence analysis in combination with physiological and chemotaxonomic data, strain CC-AFH3T represents a novel species in a new genus, for which we propose the name Noviherbaspirillum malthae gen. nov., sp. nov.; the type strain of Noviherbaspirillum malthae is CC-AFH3T ( = BCRC 80516T = JCM 18414T). We also propose the reclassification of Herbaspirillum soli , Herbaspirillum aurantiacum , Herbaspirillum canariense and ‘Herbaspirillum psychrotolerans’ as Noviherbaspirillum soli comb. nov. (type strain SUEMI10T = LMG 26149T = CECT 7840T), Noviherbaspirillum aurantiacum comb. nov. (type strain SUEMI08T = LMG 26150T = CECT 7839T), Noviherbaspirillum canariense comb. nov. (type strain SUEMI03T = LMG 26151T = CECT 7838T) and Noviherbaspirillum psychrotolerans comb. nov. (type strain PB1T = DSM 26001T = LMG 27282T), respectively. An emended description of Herbaspirillum seropedicae is also presented.