Huanghella arctica gen. nov., sp. nov., a bacterium of the family Cytophagaceae isolated from Arctic tundra soil

Abstract

A novel, strictly aerobic, red-pigmented, Gram-reaction-negative bacterium, designated strain R9-9T, was isolated from tundra soil collected near Ny-Ålesund, Svalbard Archipelago, Norway (78° N). The novel strain was subjected to a taxonomic study using a polyphasic approach. It grew optimally at 20–22 °C and at pH 7.0. Flexirubin-type pigments were absent. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain R9-9T represents a distinct phyletic line that reflects a novel generic status within the family Cytophagaceae . The novel strain showed relatively low 16S rRNA gene sequence similarities (<88.0 %) to members of established genera. Strain R9-9T contained summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C17 : 0 3-OH, iso-C15 : 0 and C16 : 1ω5c as its major cellular fatty acids, phosphatidylethanolamine as its main polar lipid, and MK-7 as its major respiratory quinone. The genomic DNA G+C content was 56.1 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain R9-9T is considered to represent a novel species in a new genus in the family Cytophagaceae , for which the name Huanghella arctica gen. nov., sp. nov. is proposed. The type strain is R9-9T ( = CCTCC AB 2010418T = NRRL B-59750T).