Muricauda taeanensis sp. nov., isolated from a marine tidal flat

Abstract

A novel Gram-stain-negative, heterotrophic, moderate halophilic and strictly aerobic bacterium, strain 105T, was isolated from a tidal flat of Taean in Korea. Cells were catalase- and oxidase-positive long rods that showed gliding motility. Optimum temperature, pH and salinity for the growth of strain 105T were observed at 30–37 °C, at pH 7.0–7.5, and in the presence of 2–4 % (w/v) NaCl, respectively. The major cellular fatty acids were iso-C15 : 1 G, iso-C15 : 0 and iso-C17 : 0 3-OH. Phosphatidylethanolamine and five unidentified lipids were identified as the major polar lipids. The genomic DNA G+C content of strain 105T was 42.4 mol% and MK-6 was detected as the predominant isoprenoid quinone. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 105T formed a phyletic lineage with members of the genus Muricauda . Strain 105T was most closely related to Muricauda aquimarina SW-63T (97.6 %), Muricauda beolgyonensis BB-My12T (97.5 %), Muricauda lutimaris SMK-108T (97.5 %), Muricauda ruestringensis B1T (97.3 %), Muricauda flavescens CL-SS4T (97.2 %) and Muricauda olearia (96.2 %). The DNA–DNA relatedness values of strain 105T with M. aquimarina JCM 11811T, M. beolgyonensis KCTC 23501T, M. lutimaris KCTC 22173T, M. ruestringensis DSM 13258T and M. flavescens JCM 11812T were 17.2±6.0, 8.7±2.2, 3.7±0.5, 11.0±1.9 and 7.1±1.3 %, respectively. On the basis of phenotypic and molecular features, strain 105T represents a novel species of the genus Muricauda , for which the name Muricauda taeanensis sp. nov. is proposed. The type strain is 105T ( = KACC 16195T = JCM 17757T).