Affiliation:
1. Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi 110007, India
Abstract
A Gram-negative, orange-pigmented, rod-shaped, motile and aerobic bacterial strain designated DM9T was isolated from hexachlorocyclohexane (HCH)-contaminated soil (Lucknow, India) and its taxonomic position was determined using a polyphasic approach. 16S rRNA gene sequence analysis showed that the isolate belonged to the phylum
Bacteroidetes
and confirmed its placement in the genus
Pontibacter
, with sequence similarity ranging from 93.92 to 96.21 % with other members of the genus
Pontibacter
. The major cellular fatty acids of the novel strain were iso-C17 : 0 3-OH (6.00 %), iso-C15 : 0 (21.54 %) and summed feature 4 (comprising C17 : 1 iso I/anteiso B; 32.3 %). The polar lipid profile of strain DM9T showed the presence of phosphatidylethanolamine, an unidentified aminophospholipid, two unknown aminolipids and four unknown polar lipids. Strain DM9T contained MK-7 as the predominant menaquinone and its DNA G+C content was 49.2 mol%. sym-Homospermidine was the major polyamine observed in the cell. The results obtained on the basis of phenotypic characteristics, phylogenetic analysis, biochemical and physiological tests clearly distinguished DM9T from closely related members of the genus
Pontibacter
. It is proposed that DM9T represents a novel species, Pontibacter lucknowensis sp. nov.; the type strain is DM9T ( = CCM 7955T = MTCC 11079T).
Funder
Council for Scientific and Industrial Research
University Grants Commission
Government of India
Department of Biotechnology, National Bureau of Agriculturally Important Micro-organisms, Indian Council of Agricultural Research
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
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