Affiliation:
1. Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany
2. Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V., Hans-Knöll-Institut, Jena, Germany
Abstract
A Gram-positive-staining, aerobic, endospore-forming bacterium, isolated as a contamination from an enrichment of enteric bacteria from surface water, was studied using a polyphasic taxonomic approach. 16S rRNA gene sequence similarity comparisons revealed that strain FSt3AT was grouped in the genus
Lysinibacillus
, most closely related to
Lysinibacillus xylanilyticus
XDB9T (98.1 %),
Lysinibacillus parviboronicapiens
BAM-582T and
Lysinibacillus sphaericus
DSM 28T (both 98.0 %). The 16S rRNA gene sequence similarity to other species of the genus
Lysinibacillus
was <97.5 %. The allocation to the genus
Lysinibacillus
was supported by a detailed chemotaxonomic characterization revealing a cell wall containing alanine, glutamic acid, aspartic acid and the diagnostic diamino acid lysine in a molar ratio of 1.6 : 1 : 0.9 : 0.8 (peptidoglycan type A4α), the major menaquinones MK-7 and MK-6, and polar lipids consisting of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, four unknown phospholipids, one unknown aminophospholipid and one unidentified aminolipid. The major fatty acids were iso- and anteiso-branched fatty acids. DNA–DNA hybridizations with the type strains of the most closely related species,
L. parviboronicapiens
DSM 25242T,
L. xylanilyticus
DSM 23493T and
L. sphaericus
DSM 28T, in addition to the results of physiological and biochemical tests, allowed genotypic and phenotypic differentiation of strain FSt3AT from these related species. Thus, FSt3AT represents a novel species of the genus
Lysinibacillus
, for which the name
Lysinibacillus
contaminans sp. nov. is proposed, with FSt3AT ( = CCM 8383T = DSM 25560T = CIP 110362T) as the type strain.
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
25 articles.
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