Affiliation:
1. Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka-shi, Kanagawa 237-0061, Japan
2. Section 1 Geochemical Oceanography, Office of Marine Research Department of Marine Science, Marine Works Japan Ltd, 2-15 Natsushima-cho, Yokosuka-shi, Kanagawa 237-0061, Japan
Abstract
A novel Gram-negative, aerobic, psychrotolerant, alkali-tolerant, heterotrophic and dimorphic prosthecate bacterium, designated strain TAR-001T, was isolated from deep-sea floor sediment in Japan. Cells of this strain had a dimorphic life cycle and developed an adhesive stalk at a site not coincident with the centre of the cell pole, and the other type of cell, a swarm cell, had a polar flagellum. Colonies were glossy, viscous and yellowish-white in colour. The temperature, pH and salt concentration range for growth were 2–41 °C, pH 6.5–10.0 and 1–4 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences confirmed that strain TAR-001T belongs to the family
Caulobacteraceae
of the class
Alphaproteobacteria
, and lies between the genus
Brevundimonas
and the genus
Caulobacter
. Levels of similarity between the 16S rRNA gene sequence of strain TAR-001T and those of the type strains of
Brevundimonas
species were 93.3–95.7 %; highest sequence similarity was with the type strain of
Brevundimonas diminuta
. Levels of sequence similarity between those of the type strains of
Caulobacter
species were 94.9–96.0 %; highest sequence similarity was with the type strain of
Caulobacter mirabilis
. The G+C content of strain TAR-001T was 67.6 mol%. Q-10 was the major respiratory isoprenoid quinone. The major fatty acids were C18 : 1ω7c and C16 : 0, and the presence of 1,2-di-O-acyl-3-O-[d-glucopyranosyl-(1→4)-α-d-glucopyranuronosyl]glycerol suggests strain TAR-001T is more closely to the genus
Brevundimonas
than to the genus
Caulobacter
. The mean DNA–DNA hybridization levels between strain TAR-001T and the type strains of two species of the genus
Brevundimonas
were higher than that of the genus
Caulobacter
. On the basis of polyphasic biological features and the 16S rRNA gene sequence comparison presented here, strain TAR-001T is considered to represent a novel species of the genus
Brevundimonas
, for which the name
Brevundimonas
abyssalis sp. nov. is proposed; the type strain is TAR-001T ( = JCM 18150T = CECT 8073T).
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
27 articles.
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