Methylophaga aminisulfidivorans sp. nov., a restricted facultatively methylotrophic marine bacterium

Abstract

A novel restricted facultatively methylotrophic marine strain, MPT, possessing the ribulose monophosphate pathway of C1-carbon compound assimilation was isolated from a seawater sample obtained from Mokpo, South Korea. The novel isolate is aerobic, Gram-negative, asporogenous and a non-motile short rod. It grows well on methanol, methylated amines, dimethylsulfide and DMSO. Optimal growth occurs with 3 % NaCl at 30 °C and pH 7.0. Fructose is utilized as a multicarbon source. Growth factors are not required and vitamin B12 does not stimulate growth. The cellular fatty acid profile of the novel strain consists primarily of straight-chain saturated C16 : 0 and unsaturated C16 : 1 acids. The major ubiquinone is Q-8. The dominant phospholipids are phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content is 44.9 mol% (T m). Based on 16S rRNA gene sequence analysis and DNA–DNA relatedness (25–41 %) with the type strains of marine methylotrophs belonging to the genus Methylophaga, it is suggested that isolate MPT represents a novel species, Methylophaga aminisulfidivorans sp. nov. (type strain MPT=KCTC 12909T=VKM B-2441T=JCM 14647T).